2024流程更改记录
产品部<marketing@genepioneer.com>
客户部<caretaker@genepioneer.com>
项目管理<run@genepioneer.com>
信息部-周向阳<zhouxy@genepioneer.com>
信息部-徐雷<xul@genepioneer.com>
信息部-于杰<yuj@genepioneer.com>
新增新的程序
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xxxxxxxxxx20240816
/share/nas1/yuj/script/Genome/1plot_genome_structure.py
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xxxxxxxxxx/share/nas2/zhangwanruo001/project/2024/chloroplast_genomics/GP-20231122-7371-1_xizangzangyiyaodaxue_mimapanduo_1sample_ximalayadahuang_yelvti/ref_adv/02_IR.py
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遗传多样性structureHarvester
/share/nas2/zhouxy/pipline/marker/snp/current/snp-diversity.pl
中structureHarvester
这一步用到的在线网站打不开,换成本地r语言绘图/share/nas1/yuj/script/diversity/deltaK_plot.r
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20240113
/share/nas1/yuj/script/chloroplast/advance/kaks/merged.py
增加kaks的汇总程序,用于绘制柱状图 -
20240520 增加
/share/nas1/yuj/script/chloroplast/annotation/draw_line_depth_v2.py
版本2,用于一个深度文件里有多个基因组的绘制 -
20240529 增加
/share/nas1/yuj/script/chloroplast/advance/calculate_pi.py all.fasta.line
滑窗计算全基因组的pi -
20240531 增加
/share/nas1/yuj/script/chloroplast/advance/pi/full_genome_pi_plot.py
绘制全基因组pi值折线图 -
20240628
/share
新增查找反向重复区的脚本,兼顾速度和准确率 -
20240718
/share/nas1/yuj/script/chloroplast/advance/pi/cp_full_genome_pi_plot.py
新增叶绿体全基因组pi分析的绘图 -
20240718
/share/nas1/yuj/script/chloroplast/advance/pi/mt_full_genome_pi_plot.py
新增动物线粒体全基因组pi分析的绘图 -
20240718
/share/nas1/yuj/script/chloroplast/advance/pi/cp_full_gene_pi_plot.py
显示大于阈值的基因 -
20240723
/share/nas1/yuj/script/Genome/ensembl_data/downfasta.py
&/share/nas1/yuj/script/Genome/ensembl_data/downgff.py
ensembl数据库批量获取链接 -
20240725
/share/nas1/yuj/script/Genome/split_chromosome_arms.py
根据着丝粒分割染色体 -
20240730
/share/nas1/yuj/script/Genome/extract_gff_annotations.sh
提取指定区间的gff -
20240807
/share/nas1/yuj/script/chloroplast/advance/pi/mt_full_gene_pi_plot.py
pi显示动物线粒体基因 -
20240816
/share/nas2/yuj/project/2024/re-sequencing/GP-20240708-8721_20240726/T-DNA/pipline/ZH310-2/find.py
从gff中寻找插入位点附近的基因 -
20240829
/share/nas1/yuj/script/chloroplast/annotation/repeats_ssr_lsr_plot/plot_ssr.py
新增单样品的ssr柱状图 -
20240903
/share/nas1/yuj/script/chloroplast/advance/pi/position_mapping.py
新增程序,获取比对前后位置对应关系,以及比对后基因所处位置 -
20240905
/share/nas1/yuj/script/chloroplast/advance/kaks/boxplot_kaks.R
新增动物线粒体kaks的箱线图 -
20240906
/share/nas1/yuj/script/chloroplast/annotation/repeats_ssr_lsr_plot/repeat_plot_bar.py
与perl程序原版对比,去掉多余线段,拆分为两张图 -
0906 /share/nas1/yuj/program/mt2/annotation/pipline/src/plot_RNAedit_nu.R
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0906
/share/nas1/yuj/script/chloroplast/advance/pi/plmt_plot.pi.no.ir.py
新增植物线粒体pi图,对比原版修改部分参数 -
0911
/share/nas1/yuj/script/fungi/annotation/fungi_parse_info_table.py
新增真菌解析mitos2结果程序 -
0914
/share/nas1/yuj/script/fungi/advance/pi/fu_plot.pi.no.ir.py
新增真菌线粒体基因pi折线图绘制,考虑取同一基因最大值绘图 -
1011
for i in *; do python /share/nas1/yuj/project/re-sequencing/GP-20230327-5799_20230519/202307-second/i1/analysis_dir/v3.py $i/analysis_dir/vcf_filter/samples.pop.snp.filter.recode.vcf > $i.region.txt && python /share/nas1/yuj/project/re-sequencing/GP-20230327-5799_20230519/202307-second/i1/analysis_dir/filter.py -r $i.region.txt -g $i/data/*.gff -o $i.matched_output.txt && grep "ID=gene-" $i.matched_output.txt > $i.filter_gene.txt & done
重测序答疑 -
1030
/share/nas1/yuj/script/chloroplast/advance/kaks/scatter_plot_kaks_values.py
新增kaks的散点图绘制
修改已有程序
- 202403 当重测序流程中,富集分析因为前后ID不一致(缺少gene-前缀)没有生成图时,使用sh /share/nas1/yuj/pipline/reseq/v1.2/script/variant_gene.sh该命令解决
- 202404 修改
/share/nas1/yuj/pipline/pop-pip/v1.1/html_report/pop_report2html.pl
,当群体指标统计目录不存在时,跳过该部分 - 20240426 当染色体结构变异文件夹不存在时去除报告相关内容
/share/nas1/yuj/pipline/reseq/v1.2/re-report_html_for_lumpy_sv.pl
- 20240508
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/mt_pip_dir.pl
当存在3代覆盖度就用3代图片,否则用2代 - 20240508
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.3dai.pl
修改对应文本描述 - 20240508
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/annotation/mt_annotation_pip.pl
将repeat命令部分写入文件 - 20240508
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/mt_pip_dir.pl
优化高级分析目录生成的代码逻辑 - 20240528 修改ir.py程序,考虑比对中有gap时的序列长度获取
- 20240531
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/mt_pip_dir.pl
修改cgview的判断方式 - 20240531
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.pl
增加pi分析的报告内容,重新添加snp、indel - 20240531
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/mt_pip_dir.pl
增加全基因组的pi分析 - 20240605
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/phytree/raxml_phytree.pl
增加-automated1参数,使trimal生效 - 20240617
/share/nas1/yuj/pipline/reseq/v1.2/script/03vcf/VariationDraw_kb.pl
修改显示单位,以Kb计算 - 20240625
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/assembly/cp.assembly.pip.pl
修改组装软件为getorganelle - 20240626
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti.pl
修改报告对应的组装描述 - 20240627
/share/nas1/yuj/pipline/reseq/v1.2/re-report_html_for_lumpy_sv.pl
恢复报告中结构变异的部分 - 20240718 ``叶绿体报告增加全基因组pi分析的图片及说明
- 20240722
/share/nas1/yuj/program/chloroplast/assembly/draw_line_depth.pl
设置y轴最大值为max depth+200 - 20240812
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti_advance.pl
完善进化树枝长的描述,增加tmp文件夹的删除 - 20240814
/share/nas1/yuj/script/chloroplast/advance/pi/cp_full_gene_pi_plot.py
新增一些绘图参数 - 20240822 删除原
/share/nas1/yuj/script/chloroplast/advance/pi/cp_full_genome_pi_plot.py
程序,将功能合并入/share/nas1/yuj/script/chloroplast/advance/pi/cp_full_gene_pi_plot.py
程序并重命名为cp_full_genome_pi_plot.py
- 20240828
/share/nas1/yuj/script/chloroplast/phytree/cp_extract_and_mafft_V2.0.py
完善修改cds列表,去除错误的基因名 - 20240828
/share/nas1/yuj/script/chloroplast/phytree/cp_from_gbk_get_cds_V4.0.py
更正输出的CDS名,增加rrna的输出 - 20240828
/share/nas1/yuj/program/chloroplast/kaks/boxplot_kaks.R
对比原版,修改长宽,加上最大值横线 - 20240830
/share/nas1/yuj/script/chloroplast/phytree/cp_from_gbk_get_cds_V4.0.py
增加寄生植物的处理 - 20240830
/share/nas1/yuj/script/chloroplast/advance/pi/mt_full_genome_pi_plot.py
调整动物线粒体pi绘图参数 - 20240830
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.pl
去除报告生成后的tmp文件夹 - 20240830
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.3dai.pl
修改线粒体全基因组pi分析的描述 - 20240903
/share/nas1/yuj/script/chloroplast/advance/pi/mt_full_gene_pi_plot.py
修改曲线为折线,修改基因标签的样式,修改图片标题名 - 20240905
/share/nas1/yuj/script/chloroplast/advance/kaks/merged.py
修改kaks的汇总程序,根据不同参数输出不同的文件,以便于绘图 - 20240909
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/cp_pip_dir.pl
叶绿体增加kaks箱线图到结果目录 - 20240909
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti.pl
叶绿体报告增加kaks箱线图及其描述 - 20240909
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti_advance.pl
高级分析报告新增内容同上 - 20240909
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/cp_pip_dir_only_advance.pl
叶绿体仅高级分析目录增加kaks箱线图 - 0912
/share/nas1/yuj/program/mt2/coline/src/draw_collinearity.pl
修改植物线粒体共线性图的颜色 - 0913
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/annotation/etc/plot_ogdraw_cpgenome_genbank/conf/OGDraw_chondriom_set.xml
增加真菌线粒体rps3基因的绘图参数 - 0914
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.pl
增加线粒体同源基因pi值及kaks箱线图描述 - 0914
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/mt_pip_dir.pl
增加线粒体kaks箱线图和基因pi值 - 0920
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/annotation/bin/chloroplast_annotaion.pl.v2.1.pl
修改样式2深度线性图的程序路径,为v1版本 - 0925
/share/nas1/yuj/script/chloroplast/advance/pi/plmt_plot.pi.no.ir.py
按客户要求修改字体以及字号大小 - 0926
/share/nas1/yuj/script/chloroplast/advance/pi/plmt_plot.pi.no.ir.py
根据pi最大值自动设定Y轴刻度,且Y轴线段终止于最后刻度 - 0927
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.pl
1.修改为“当箱线图存在时,生成对应部分”;2.修改共有cds构建进化树的方法描述 - 0927
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti_sandai.pl
1.修改叶绿体2+3代数据组装方法描述;2.增加3代覆盖度图片;3.运行结束删除tmp文件夹 - 0927
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/cp_pip_dir.pl
增加3代覆盖度图片整理 - 0930
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti.pl
1.修改为“当箱线图存在时,生成对应部分”;2.修改全基因组最大似然法进化树的方法描述 - 0930
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/html_report/report2xml.yelvti_advance.pl
1.修改为“当箱线图存在时,生成对应部分”;2.修改全基因组最大似然法进化树的方法描述;3.修改kaks的统计表生成 - 0930
/share/nas1/yuj/pipline/marker/snp/v1.0/Web_Report/snp_Web_Report.pl
1.修改为当GenAlEx结果存在时,生成对应部分;2.去除指纹图谱
202410
- 1017
/share/nas1/yuj/program/mt2/annotation_for_multi_seq/pipline/repeat/mt2_repeat.pl
修改mt重复序列圈图的ssr参数以供叶绿体分析用 - 1022
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/mt_pip_dir.pl
补全四种进化树的整理结果 - 1022
/share/nas6/pub/pipline/genome-assembly-seq/mitochondrial-genome-seq/v2.0/html_report/report2xml.xianliti.pl
补全四种进化树的方法描述 - 1023
/share/nas1/yuj/script/chloroplast/get_ass_cfg.py
提供样品名映射文件,自动将送样编号替换为拉丁名 - 1023
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/assembly/cp.assembly.pip.pl
移除组装完成后的其他步骤,缩短流程时间 - 1028
/share/nas1/yuj/pipline/reseq/v1.2/re-report_html_for_lumpy_sv.pl
参考基因组无gff时,跳过未生成结果部分的报告描述 - 1028
/share/nas1/yuj/pipline/reseq/v1.2/script/02variant/snp_ver.stat.pl
考虑颠换为0时,Ti/Tv赋值为null,避免程序出错 - 1029
/share/nas1/yuj/pipline/genetic_diversity_pip/v1.0/script/10pts/pca/drawPCA_gtca.R
按客户要求,增加PCA图字体大小
202411
- 1101
/share/nas6/pub/pipline/genome-assembly-seq/chloroplast-genome-seq/v1.2/asmqc/src/cp2circos.pl
修改叶绿体覆盖度的统计,使最大值可以显示 - 1105
/share/nas1/yuj/script/chloroplast/annotation/cp_add_gene_seq.py
解决一些特殊基因的序列获取顺序,如rps12